Matrigel Matrix | Extracellular Matrix | Corning

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Corning® Matrigel® Matrix

Corning® Matrigel® Matrix

Corning® Matrigel® Matrix

matrigel

10,000 Citations and Counting

10,000 Citations and Counting

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Corning Matrigel Bulk Packs

The more Matrigel matrix you buy, the more you save.

The more you buy, the more you save.

The leading ECM-based hydrogel for 2D and 3D cell culture application is now available in convenient bulk packaging.

The leading ECM-based hydrogel for 2D and 3D cell culture application is now available in convenient bulk packaging.

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Overview

Corning Matrigel Matrix

Cells behave better on Corning Matrigel matrix—the original, trusted extracellular matrix (ECM).

Nearly 30 years ago, researchers were looking for a way to grow mouse sarcoma cells. The solution? The development of Corning Matrigel matrix, a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Today, this natural ECM-based hydrogel is among the most widely used models for 2D and 3D cell culture in vitro, enabling you to:

  • Improve the attachment and differentiation of both normal and transformed anchorage-dependent epitheliod cells, as well as other cell types.
  • Better mimic in vivo environments for 2D and 3D cell culture applications.

Free Download

Request a copy of the Ultimate Guide to Matrigel Matrix – a go-to resource packed with a plethora of resources from top tips and facts to expert advice on Matrigel Matrix.

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About
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Characteristics of Matrigel Matrix

  Standard Formulation High Concentration (HC) Growth Factor Reduced (GFR) Phenol Red Free

hESC-qualified
Application

Suitable for culture of polarized cells, such as epithelial cells.

Promotes differentiation of many cell types, including hepatocytes, neurons, beta-islets, mammary epithelial, endothelial, and smooth muscle cells.

Higher protein concentration provides greater matrix stiffness and scaffold integrity.

Suitable for in vivo cell delivery applications for improved cell engraftment and augmentation of solid tumor formation.

Suited for applications where a more highly defined basement membrane preparation is desired.

Available in standard, Phenol red-free, and GFR formulations.

 Suitable for assays that require color detection (e.g., colorimetric, fluorescence).Available in standard, GFR, and HC formulations. Pre-screened for compatibility with mTeSR®1 medium by Stem Cell Technologies, providing the reproducibility and consistency essential for human embryonic and induced pluripotent feeder-free stem cell culture.

Source

Mouse

Mouse

Mouse

Mouse

Mouse

Protein Concentration

8-12 mg/mL

 

18-21 mg/mL

 

8-12 mg/mL

 

8-12 mg/mL

 

8-12 mg/mL

 

Shelf Life

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

Vialed Formats

(Cat. No./Qty.)

356234 | 5 mL

354234 | 10 mL

356235 | 5 x 10 mL

356237 | 10 mL (Phenol red-free)

354248 | 10 mL

354262 | 10 mL (Phenol red-free)

354263 | 10 mL (GFR)

356230 | 5 mL (Standard)

354230 | 10 mL (Standard)

354263 | 10 mL (HC)

356231 | 10 mL (Phenol red-free)

356237 | 10 mL (Standard)

354262 | 10 mL (HC)

356231 | 10 mL (GFR)

354277 | 5 mL

 

BioCoatTM Options

Plates: 6 well, 12 well, 24 well, 48 well, 96 well

Inserts: for 24 well plates

Dishes: 35 mm, 60 mm, 100 mm

        
Features

Unique features of Matrigel matrix include:

Unique features of Matrigel matrix include:

  • Proven and trusted since 1985; most extensively referenced basement membrane matrix.
  • Wide selection of basement membranes; used in diverse applications, from cancer and stem cell research to neurobiology.
  • Manufactured under stringent quality control practices that exceed industry standards, ensuring protein consistency and superior product performance.
  • Rigorous validation for functionality.
  • Certified LDEV-free.
  • Proven and trusted since 1985; most extensively referenced basement membrane matrix.
  • Wide selection of basement membranes; used in diverse applications, from cancer and stem cell research to neurobiology.
  • Manufactured under stringent quality control practices that exceed industry standards, ensuring protein consistency and superior product performance.
  • Rigorous validation for functionality.
  • Certified LDEV-free.
Cells
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Compatible Cell Types

Primary Cells

 

Cell Lines (Transformed or Transfected)

Stem and Progenitor Cell Expansion

In Vitro Differentiation of Pluripotent Stem Cells

In Vitro Differentiation of Adult Stem Cells

Aortic endothelial cells, BAEC

Bile duct cells (epithelial)

Brain microvessel (endothelial)

Cardiomyocytes; cardiac (endothelium, progenitor cells)

Colonocytes (epithelial)

Dorsal root ganglia

Embryonic cortical neurons

Embryonic sympathetic neurons

Hepatocytes

Hippocampal neurons

HUVEC (endothelial)

HVSMC

Keratinocytes

Mammary epithelial cells; breast cells (luminal, myoepithelial and endothelial)

Microvascular, BME (endothelial)

Mouse splenic T-Cells

Muscle cells, myoblasts, myogenic cells, myotubes

Oligodendrocytes (glial; precursors)

Pancreatic islet, neonatal (3- to 5-day-old) rat islets of Langerhans

Parotid acinar cells

Peripheral blood mononuclear cells

Schwann cells (glial)

Sertoli cells (spermogenic)

Skeletal muscle cells (myocytes, myotubes)

Smooth muscle cells (endothelial, vascular)

Urothelial cells

Breast cancer cells (established cell lines)

C2C12 (myoblast)

CHO, CHO-1, CHO-K1 (epithelial, endothelial, transfected fusion protein)

COS-7 (fibroblast, transfected)

Dorsal Root Ganglia (transfected)

H1299 (transfected- human non-small cell lung carcinoma cell line)

HEK-293 (transfected, epithelial), EcoPack2™-293, HEK-SRAtet cells, Living Colors HEK-ZsGreen proteasome sensor (transfected)

HepG2 (hepatocyte)

HT-1080 (epithelial)

hFOB 1.19, MG63 (osteoblast cell lines)

L929 (fibroblast, transfected)

LnCAP (prostate cancer cell line)

MCF-10A (epithelial

MDA-MB-231

MDA-MB 435

NIH/3T3, 3T3 (fibroblast)

PC-3, PC-12

RTG-2 (rainbow trout gonad cells)

SH-SY5Y

 

Human embryonic stem cell (hESC)

Human induced pluripotent stem cell (hiPSC)

 

hESC (cerebral organoid model)

hESC (pancreatic)

hESC, hiPSC (cardiomyocytes)

hESC, hiPSC, mESC (Germ Cell Layers: ectoderm, mesoderm, endoderm; hematopoietic progenitor; definitive differentiation; cardiomyocytes)

hESC, hiPSC, mESC, miPSC (endothelial)

hESC, hiPSC (intestinal organoids)

hESC, hiPSC (neuronal)

hESC, hiPSC (smooth muscle)

hESC, mESC (lung epithelial)

hESC, mESC, rESC (hepatocyte, hepatocyte-like)

Human NPCs (differentiation to neuronal cells)

hPSCs, mPSCs (renal progenitor cells, renal tubular cells, endoderm)

mESC (hematopoietic)

mESC, Chicken (cardiomyocytes)

mESC, rESC, miPSC (neuronal, progenitor)

mPSCs (inner ear sensory epithelia)

 

hADSCs; Adipose (endothelial)

Colon (epithelial organoids)

Hair Follicle (melanocytes, neurons, smooth muscle)

Intestinal (organoids, crypt-villus)

 
Lot Selection Tool

Reserve a consistent supply of Matrigel Matrix

Reserve a consistent supply of Matrigel Matrix

Learn More

Use the online Corning Matrigel Lot Selection and Reserve Tool to:

  • Set up a lot reserve, which simplifies storage and supply chain resources.
  • Find a production lot with similar specifications to the previously requested lot number.

Use the online Corning Matrigel Lot Selection and Reserve Tool to:

  • Set up a lot reserve, which simplifies storage and supply chain resources.
  • Find a production lot with similar specifications to the previously requested lot number.
Learn More
Technical Resources for ECMs

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From review articles and brochures to protocols, application notes, FAQs and more, we have all the ECM resources to get your research up and running.

From review articles and brochures to protocols, application notes, FAQs and more, we have all the ECM resources to get your research up and running.

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Applications

Corning Matrigel matrix is effective for the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid and other cell types. These include neurons, Sertoli cells, chick lens, vascular endothelial cells, and hepatocytes.

Applications include:

  • Influencing gene expression in adult rat hepatocytes
  • 3D culture in mouse and human mammary epithelial cells
  • In vivo peripheral nerve regeneration
  • Metabolism and toxicology studies
  • The development of several types of tumor cell invasion assays
  • The in vitro and in vivo study of angiogenesis
  • In vivo propagation of human tumors in immunosuppressed mice
  • The maintenance of human embryonic stem cells and induced pluripotent stem (hiPS) cells
  • Neuronal differentiation of hiPS cells

Matrigel Matrix Quality Control Specifications

  • Mouse colonies routinely screened for pathogens via mouse antibody production (MAP) testing
  • LDEV-free EHS tumor
  • Extensive PCR testing performed on a number of pathogens, including LDEV, ensuring strict control of raw materials used during manufacturing
  • Tested and found negative for the presence of bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Gel stability tested for a period of 14 days at 37°C
  • Biological activity determined for each lot using a neurite outgrowth assay; chick dorsal root ganglia, plated on a 1.0 mm layer of Corning Matrigel matrix, required to generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

How to Buy

Purchasing Options

Order Online

Order Online

It’s easy to purchase Matrigel matrix online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

It’s easy to purchase Matrigel matrix online. Order direct with a credit card or use a purchase order if you have an account with Corning.

Distributors

Distributors

Contact your authorized distributor to order Matrigel matrix.

Contact your authorized distributor to order Matrigel matrix.

Customer Service

Customer Service

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

Applications and Use

Matrigel Matrix in Action

Featured Resources

Featured Resources

3D Matrices

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration.

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Protocols

Protocols for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro.

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What’s New

Featured Products, Resources, and More

Corning Matrigel Matrix FAQs

Corning Matrigel Matrix FAQs

Get answers to commonly asked questions about Corning Matrigel matrix.

Corning Matrigel Matrix Frequently Asked Questions

From growth factor concentration to cell recovery and storage, learn how to optimize your cell culture with Corning Matrigel matrix.

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Corning Matrigel Lot Selection and Reserve Tool

Corning Matrigel Lot Selection and Reserve Tool

Make sure your experiments are as consistent as possible. Reserve a consistent supply of Matrigel.

Extracellular matrices are complex biological reagents, and, like all biologically derived reagents, they may be subject to lot-to-lot variation. Our stringent quality control and manufacturing practices minimize variation. In addition, you can use the online Corning Matrigel Lot Selection and Reserve Tool to:

  • Set up a lot reserve, which simplifies storage and supply chain resources.
  • Find a production lot with similar specifications to the previously requested lot number.
Learn More

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