Collagen for Cell Culture

Collagen is an extracellular matrix (ECM) that enables researchers to mimic in vivo environments for 2D and 3D cell culture applications through culturing a variety of cell types to promote adhesion, growth, and/or differentiation.

Improve Cell Culture Success by Promoting Adhesion, Growth, & Differentiation with Corning Collagen

Corning has developed a broad range of collagen types derived from multiple species to support your diverse needs. hese high quality collagen surfaces undergo rigorous quality control testing to ensure consistent results. Corning Collagen surfaces can be applied as a thin coating, and in some cases, as a gel on a wide range of cultureware, providing the flexibility you need for diverse cell-based assays and collagen tissue engineering.

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Characteristics and Applications of Collagen

Rat/Human/Bovine

Characteristics and Applications

	Rat Tail Collagen I, sterile filtered	Rat Tail Collagen I High Concentration, sterile filtered	Human Collagen I	Bovine Collagen I	Bovine Collagen II, sterile filtered
Vialed Formats (Cat. No./Qty.)	354236 \| 100 mg 356236 \| 1 g (10 x 100 mg)	354249 \| 100 mg	354243 \| 0.25 mg 354265 \| 10.0 mg	354231 \| 30 mg	354257 \| 5 mg
Applications	Suitable for a thin layer on tissue culture surfaces to enhance cell attachment and proliferation or as a gel to promote expression of cell-specific morphology and function. Commonly used to culture endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.	High concentration provides greater matrix stiffness and scaffold integrity; suitable for 3D cell culture applications.	Suitable for a thin layer on tissue culture surfaces to enhance cell attachment and proliferation	Preparation contains native collagen molecules with a small amount of nicked or shortened sequences due to pepsin treatment.	Suitable for attachment and differentiation of chondrocytes. Can also be used as an in vivo model in rats and mice for arthritis studies
Source	Rat tail	Rat tail	Human placenta	Bovine	Bovine
Protein Concentration	3-4 mg/mL in 0.02 N acetic acid	8-11 mg/mL in 0.02 N acetic acid	2-4 mg/mL frozen in 2 mM Hydrochloric acid	2.9-3.1 mg/mL in 0.01 N hydrochloric acid	2.9-3.3 mg/mL, frozen in 15 mM acetic acid
Shelf Life	2 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	1 year from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.
BioCoat Options	Multiple well plates: 6 well, 12 well, 24 well, 48 well, 96 well, 384 well Dishes: 35 mm, 60 mm, 100 mm, 150 mm Flasks: T-25, T-75, T-150, T-175 (vented cap) Cover slip: 22 mm, round Culture slides: 4 well and 8 well Custom coating options available	Custom coating options available	Custom coating options available	Custom coating options available	Custom coating options available

Rat Tail Collagen I, sterile filtered

Rat Tail Collagen I High Concentration, sterile filtered

Human Collagen I

Bovine Collagen I

Bovine Collagen II, sterile filtered

Vialed Formats (Cat. No./Qty.)

354236 | 100 mg

356236 | 1 g (10 x 100 mg)

354249 | 100 mg

354243 | 0.25 mg

354265 | 10.0 mg

354231 | 30 mg

354257 | 5 mg

Applications

Suitable for a thin layer on tissue culture surfaces to enhance cell attachment and proliferation or as a gel to promote expression of cell-specific morphology and function.

Commonly used to culture endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.

High concentration provides greater matrix stiffness and scaffold integrity; suitable for 3D cell culture applications.

Suitable for a thin layer on tissue culture surfaces to enhance cell attachment and proliferation

Preparation contains native collagen molecules with a small amount of nicked or shortened sequences due to pepsin treatment.

Suitable for attachment and differentiation of chondrocytes.

Can also be used as an in vivo model in rats and mice for arthritis studies

Source

Rat tail

Human placenta

Bovine

Protein Concentration

3-4 mg/mL in 0.02 N acetic acid

8-11 mg/mL in 0.02 N acetic acid

2-4 mg/mL frozen in 2 mM Hydrochloric acid

2.9-3.1 mg/mL in 0.01 N hydrochloric acid

2.9-3.3 mg/mL, frozen in 15 mM acetic acid

Shelf Life

2 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

2 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

2 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

2 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

1 year from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

BioCoat Options

Multiple well plates: 6 well, 12 well, 24 well, 48 well, 96 well, 384 well

Dishes: 35 mm, 60 mm, 100 mm, 150 mm

Flasks: T-25, T-75, T-150, T-175 (vented cap)

Cover slip: 22 mm, round

Culture slides: 4 well and 8 well

Custom coating options available

Human/Mouse/Porcine

Characteristics and Applications, Continued

	Human Collagen III	Human Collagen IV	Mouse Collagen IV	Human Collagen V	Human Collagen VI
Vialed Formats (Cat. No./Qty.)	354244 \| 0.25 mg	354245 \| 0.25 mg	354233 \| 1 mg 356233 \| 10.0 mg (10 x 1 mg)	354246 \| 0.25 mg	354246 \| 0.25 mg
Applications	Found in several connective tissues including the dermis of young organisms, human skin, and cornea. It can be used as a thin coating on tissue culture surfaces to promote cell attachment and to modulate cell behavior.	A ubiquitous component of the basement membrane. The sheet-like matrix is found in close proximity to epithelial, muscle, and nerve cells. Plays a role in the regulation of cell growth, differentiation, and tissue formation.	A ubiquitous component of the basement membrane. The sheet-like matrix is found in close proximity to epithelial, muscle and nerve cells. Plays a role in the regulation of cell growth, differentiation, and tissue formation.	Found in whole placenta, amnion, chorion, and cornea. Suitable as a thin coating on tissue culture surfaces to study Collagen V effects on cell behavior.	A large, multidomain ECM. Its heterotrimeric chains assemble into microfibrillar networks via tetramerization and end-to-end association. Generally used as a coating but may also be added to cell culture media.
Source	Human placenta	Human placenta	Engelbreth-Holm- Swarm lathrytic mouse tumor	Human placenta	Human placenta
Protein Concentration	0.9-1.1 mg/mL in 10 mM Acetic acid	0.5-1 mg/mL, frozen in 10 mM Acetic acid	0.2-1 mg/mL, frozen in 0.05 M Hydrochloric acid	0.8-1 mg/mL, frozen in 10 mM Acetic acid	0.3-0.5 mg/mL frozen in 1 M Sodium Chloride, 1.25 mM Tris, pH 8.0
Shelf Life	2 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	2.5 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	2 years from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.	1.5 year from date of manufacture. Date of expiration is located on lot-specific certificate of analysis.
BioCoat Options	Custom coating options available	Custom coating options available	Multiwell Plates: 6-well, 24-well, 96-well Dishes: 35 mm, 60 mm, 100 mm Flasks: T-25, T-75, T-175 Culture Slides: 4- well and 8-well Inserts: for 6-well and 24-well plates Custom coating options available	Custom coating options available	Custom coating options available

Human Collagen III

Human Collagen IV

Mouse Collagen IV

Human Collagen V

Human Collagen VI

Vialed Formats (Cat. No./Qty.)

354244 | 0.25 mg

354245 | 0.25 mg

354233 | 1 mg 356233 | 10.0 mg (10 x 1 mg)

354246 | 0.25 mg

Applications

Found in several connective tissues including the dermis of young organisms, human skin, and cornea.

It can be used as a thin coating on tissue culture surfaces to promote cell attachment and to modulate cell behavior.

A ubiquitous component of the basement membrane.

The sheet-like matrix is found in close proximity to epithelial, muscle, and nerve cells.

Plays a role in the regulation of cell growth, differentiation, and tissue formation.

A ubiquitous component of the basement membrane.

The sheet-like matrix is found in close proximity to epithelial, muscle and nerve cells.

Plays a role in the regulation of cell growth, differentiation, and tissue formation.

Found in whole placenta, amnion, chorion, and cornea.

Suitable as a thin coating on tissue culture surfaces

to study Collagen V effects on cell behavior.

A large, multidomain ECM.

Its heterotrimeric chains assemble into microfibrillar networks via tetramerization and end-to-end association.

Generally used as a coating but may also be added to cell culture media.

Source

Human placenta

Engelbreth-Holm- Swarm lathrytic mouse tumor

Human placenta

Protein Concentration

0.9-1.1 mg/mL in 10 mM Acetic acid

0.5-1 mg/mL, frozen in 10 mM Acetic acid

0.2-1 mg/mL, frozen in 0.05 M Hydrochloric acid

0.8-1 mg/mL, frozen in 10 mM Acetic acid

0.3-0.5 mg/mL frozen in 1 M Sodium Chloride, 1.25 mM Tris, pH 8.0

Shelf Life

2 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

2.5 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

2 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

2 years from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

1.5 year from date of manufacture.

Date of expiration is located on lot-specific certificate of analysis.

BioCoat Options

Custom coating options available

Multiwell Plates: 6-well, 24-well, 96-well

Dishes: 35 mm, 60 mm, 100 mm

Flasks: T-25, T-75, T-175

Culture Slides: 4- well and 8-well

Inserts: for 6-well and 24-well plates

Custom coating options available

Quality

Quality Control

Testing for quality control varies by collagen type:*

Purity > 90% by SDS-PAGE
Tested for ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells
Tested and found negative for the presence of bacteria, fungi, and mycoplasma

* Not all tests are completed on all collagens.

Testing for quality control varies by collagen type:*

Purity > 90% by SDS-PAGE
Tested for ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells
Tested and found negative for the presence of bacteria, fungi, and mycoplasma

* Not all tests are completed on all collagens.

Products

Collagen Coatings for Cell Culture

Collagen I

Use Corning Collagen I surface to culture endothelial cells, hepatocytes, muscle cells, breast carcinoma cells, platelets, transfected cell lines, among many others.

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Collagen II

Support chondrocyte adhesion, attachment, and differentiation, and possibly influence the differentiated phenotype of chondrocytes, with Corning bovine Collagen II.

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Collagen III

Enhance the research of collagen fibrillogenesis in normal cardiovascular development with Corning human Collagen III.

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Collagen IV

Successfully grow epithelial, endothelial, muscle, and nerve cells and support PC12 adhesion and proliferation, and more, with Corning human and mouse Collagen IV.

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Collagen V

Collagen V is found in placenta, amnion, chorion, and cornea. Modulate the proliferative response of endothelial cells with Corning human Collagen V.

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Collagen VI

Enhance the success of specialized tasks in tissue organization and cell functionality through the distribution pattern, unique structure, and expression profiles of Corning human Collagen VI.

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BioCoat™ Gelatin

Promote the attachment and growth of a variety of cell types with Corning BioCoat Gelatin cultureware. Successfully culture vascular endothelial cells, HUVEC cells, and embryonic stem cells.

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Corning® Collagen Products Frequently Asked Questions

Q: What does a collagen gel look like?

A: A Corning Collagen gel looks like an agarose gel, but it is more delicate. If touched with the end of a pipet, it will break apart
Q: How stable is a collagen gel over time?

A: The Corning Collagen gel will start to contract away from the edges of a dish after 2 weeks, so we do not suggest making a gel ahead of time.
Q: What is the storage recommendation for collagen products?

A: Storage recommendations for Corning Collagen products vary from 4°C to -70°C, depending on the collagen type. Specific information can be found on the product specification sheet. Frozen Collagen products should not be stored in a frost-free freezer. Avoid multiple freeze-thaws.
Q: What is the stability for collagen coating on a cultureware?

A: We recommend air drying Corning Collagen-coated cultureware and then storing it at 4°C in a sealed container that will help protect it from moisture. Under these conditions the coated cultureware should be stable for at least two weeks.
Q: Why is collagen not gelling?

A: Protein concentration for Collagen varies from lot-to-lot. Normalize your calculations based on protein concentrations. It is critical the pH (e.g., for Corning Collagen I rat tail) should be as recommended in the product manual for collagen gelation. Make sure the product is stored as recommended and is not expired.

Learn how to coat Corning HYPERFlask Vessels with collagen or gelatin.

Collagen Coated Cell Culture Vessels Protocol

This protocol presents the best methods to produce thin coatings of collagen or gelatin on HYPERFlask^® vessels to aid cell attachment and spreading.

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Applications and Use

Collagen in Cell Culture Applications

Cell Growth and Differentiation

Cell Growth & Differentiation

See how Corning Collagen surfaces can improve cell growth and differentiation

3D Cell Culture

Sophisticated in vitro 3D cell culture models created with Corning Collagen

Cancer Research

Achieve significant advances in cancer cell biology with Collagen Surfaces.

Cancer Disease Modeling

Further cancer research with more in vivo-like disease models.

Culture and Assay Systems

3D cell cultures can mimic the specificity of native tissue.

The Progression of Cancer

Cancer research is complex. Finding a trusted, experienced partner is simple.

Stem Cell Differentiation & Analysis

Promote successful cell differentiation & advance stem cell analysis.

How to Buy

Purchasing Corning Collagens

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It’s easy to purchase Corning collagens online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

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Distributors

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Contact your authorized distributor to order Corning Collagen surfaces.

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Customer Service

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

Collagens | Cell Culture Collagen Tissue | Corning

Corning® Collagens